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ABSTRACT MotivationFreshwater ecosystems have been heavily impacted by land‐use changes, but data syntheses on these impacts are still limited. Here, we compiled a global database encompassing 241 studies with species abundance data (from multiple biological groups and geographic locations) across sites with different land‐use categories. This compilation will be useful for addressing questions regarding land‐use change and its impact on freshwater biodiversity. Main Types of Variables ContainedThe database includes metadata of each study, sites location, sample methods, sample time, land‐use category and abundance of each taxon. Spatial Location and GrainThe database contains data from across the globe, with 85% of the sites having well‐defined geographical coordinates. Major Taxa and Level of MeasurementThe database covers all major freshwater biological groups including algae, macrophytes, zooplankton, macroinvertebrates, fish and amphibians.